The full-length reads delivered by nanopore sequencing enable the unambiguous characterisation and quantification of transcript isoforms, providing a true reflection of gene expression and comprehensive analysis of the human transcriptome.
Oxford Nanopore is the only sequencing technology offering direct sequencing of native RNA, allowing the simultaneous detection of base modifications alongside nucleotide sequence. With direct cDNA or RNA sequencing, PCR bias is also eliminated.
Scale to your requirements: all three methods of RNA sequencing (direct RNA, direct cDNA, and PCR-cDNA) are compatible across the Oxford Nanopore sequencing platforms, from the smaller-scale Flongle and MinION with outputs of 1.8 Gb and 10-30 Gb, respectively, to the higher output GridION and PromethION producing up to 150 Gb and 9.6 Tb (P48), respectively. With PCR-cDNA sequencing, obtain 7–12+ million full-length reads per MinION/GridION Flow Cell, and 21-36+ million reads per PromethION Flow Cell.