Epigentek Group Inc.  

Farmingdale,  NY 
United States
https://www.epigentek.com
  • Booth: 1634

EpiGentek provides your complete solutions for genetics, epigenetics, transcription factors & signal transduction studies. Founded in 2005, we’ve built a foundation of trusted and reliable research kits, antibodies & services. Our mission is to provide top-of-market speed and effectiveness in our technologies as we focus on developing our product portfolio to enhance the way our customers perform research.

Since our inception, our products have become some of the most recognizable on the market, including:

  • The MethylFlash™ brand of methylation kits; fast 2-hour quantitation of global DNA methylation or hydroxymethylation via a one-step ELISA method.

  • A catalog of over 20,000 quality-guaranteed antibodies with free 2-day shipping. Including conjugates, secondary antibodies, & antibody panel packs.

  • The EpiQuik™ m6A RNA Methylation Quantification Kit for 3-hour 45-minute absorbance-based quantitation of N6-methyladenosine in an ELISA-like format.

  • The all-new BilsulPlus™ Loci 5mC & 5hmC Detection PCR Kit for rapid, affordable & reliable gene-specific differentiation of 5-mC and 5-hmC using an optimized double-conversion method.

No matter what your genetic research involves, EpiGentek has a solution to help you be more precise and time-efficient in your studies.

Want to discuss your next project to see how our products or services can help? Call us toll-free in the US & Canada at 1-877-EPIGENTEK (374-4368), email us at sales@epigentek.com, or visit our website www.epigentek.com.

Brands: EpiGentek, MethylFlash, EpiQuik, BisulFlash, BisulPlus, EpiMag, ChromaFlash, EpiSonic, Epigenase, FitAmp, Methylamp, EpiNext, SuperSense


 Videos

BisulPlus Loci 5mC & 5hmC Detection PCR Kit
EpiMag 96-Well Magnetic Separator Rack

 Products

  • MethylFlash™ Global DNA Methylation ELISA Easy Kit
    Quantitate global DNA methylation by measuring levels of 5-mC via a one-step ELISA method. 2-hour protocol time, flexible 96 strip-well microplate format, highly specific to 5-mC with no cross-reactivity and low background noise....

  • The MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit is a complete set of optimized buffers & reagents to colorimetrically quantify global DNA methylation status by specifically measuring levels of 5-methylcytosine (5-mC) in a simplified, "one-step" ELISA-like reaction.

    • Fast - Entire procedure only needs 2 hours.
    • Robust - Greater "signal window" with reduced variation between replicates.
    • Convenient - Inherently low background noise.
    • Sensitive - Detection limit as low as 0.05% methylated DNA from 100 ng of input DNA.
    • Specific - High specificity to 5-mC, with no cross-reactivity to unmethylated cytosine or hydroxymethylcytosine.
    • Universal - Positive & negative controls included for detection of DNA methylation in any species from either single- or double-stranded input DNA.
    • Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate.
    • Flexible - Strip-well microplate format for manual or high throughput analysis.
  • EpiQuik™ m6A RNA Methylation Quantification Kit
    Quantitate N6-methyladenosine (m6A) in RNA in an ELISA-like format. Fast 3-hour 45-minute protocol time, high sensitivity (detection limit as low as 10 pg of m6A), flexible 96 strip-well microplate format & highly specific to m6A with no cross-reactivity....

  • The EpiQuik™ m6A RNA Methylation Quantification Kit (Colorimetric) is a complete set of optimized buffers and reagents to colorimetrically quantify N6-methyladenosine (m6A or 6mA) in RNA.

    • Colorimetric ELISA-like assay using an m6A antibody with easy-to-follow steps for convenience and speed. Rapid procedure of 3 hours and 45 minutes.
    • High sensitivity; detection limit as low as 10 pg of m6A.
    • Unique binding solution allows RNA >70 nts to be tightly bound to the wells, which enables quantification of m6A from both mRNA and ncRNA such as tRNA, rRNA, and snRNA.
    • Optimized antibody & enhancer solutions allow high specificity to m6A, with no cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA.
    • Universal positive and negative controls are included, which are suitable for quantifying m6A from any species.
    • Strip-well microplate format for manual or high throughput analysis.
    • Simple, reliable, and consistent assay conditions.
  • EpiQuik™ Nuclear Extraction Kit
    Isolate and extract nuclear proteins from cells or tissues while keeping enzymatic activity intact. Pre-optimized 1-hour protocol, high yield of 100 µg per optimal extraction, standardized procedure for consistent, reproducible results....

  • The EpiQuik™ Nuclear Extraction Kit is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins used for a variety of applications in just 60 minutes.

    The kit is also specifically designed to meet the requirements of nuclear extracts used in various other epigenetic products from Epigentek, including DNMTHDAC, and HAT assays. Some of the kit's highlighted features include the following:

    • Fast - Pre-optimized and simple step-wise 1-hour protocol.
    • Convenient – The kit includes all essential reagents to carry out a nuclear extraction.
    • High Yield – Total yield can be up to 100 µg per optimal extraction (results may vary depending on cell or tissue type).
    • Consistent - Standardized procedure for reproducible results.
    • Quality Controlled – EpiGentek guarantees the performance of the EpiQuik™ Nuclear Extraction kit in the manner described in the provided product instructions
  • BisulPlus™ Loci 5mC & 5hmC Detection PCR Kit
    Reliably detect and differentiate 5mC and 5hmC simultaneously using a small amount of input DNA. Fast 5-hour procedure with innovative method, high specificity of 5hmC discriminated from C and 5mC and other modified cytosines....

  • The BisulPlus™ Loci 5mc & 5hmC Detection PCR Kit is a complete set of optimized buffers and reagents to identify both 5mC and 5hmC on a loci- or gene-specific level by qPCR using bisulfite conversion and cytosine deaminase treatment.
     

    • Simultaneous Reading: DNA treatment is prepared simultaneously for both 5hmC and 5mC, which allows for parallel identification in a loci- or gene-specific manner simultaneously by qPCR.
    • Innovative Method: A combination of bisulfite and subsequent cytosine deaminase (APOBEC) treatment allows for tactical conversion of 5mC in order to identify 5hmC.
    • High Specificity: 5hmC is discriminated from C and 5mC as well as from other modified cytosines 5fC and 5caC.
    • Flexibility: Perform 24 reactions each for 5hmC & 5mC analysis simultaneously, or perform 48 reactions for just 5mC.
    • Fast and Streamlined Procedure: The procedure can be completed within 5 hours.
    • Complete Conversion: Completely converts C into uracil (>99%) and 5mC to thymine (>95%)
    • Broad sample suitability: Starting materials can be genomic DNA isolated from various tissue/cell samples such as fresh and frozen tissue, cultured cells from a flask or microplate, microdissection samples, paraffin-embedded tissue, biopsy, embryonic cells, plasma/serum samples, and body fluid samples, etc. DNA enriched from various enrichment reactions such as ChIP, MeDIP/hMeDIP, or exon capture may also be used as starting material.
  • EpiQuik™ Histone H3 Modification Multiplex Kit
    ELISA-like quantitation of 21 different histone H3 modification patterns on the same plate. Quick 2-hour 30-hour procedure, detection threshold as low as 0.5 ng/well, total histone H3 sets included, reliable & consistent assay conditions....

  • The EpiQuik™ Histone H3 Modification Multiplex Assay Kit (Colorimetric) is a complete set of optimized reagents to detect and quantify up to twenty-one (21) modified histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader. The kit has the following advantages and features:

    • Simultaneously screen and measure 21 different histone H3 modifications, which includes all of the most important and the most well-characterized patterns.
    • Quick and efficient procedure, which can be finished within 2 hours and 30 minutes.
    • Innovative colorimetric assay without the need for radioactivity, electrophoresis, chromatography, or expensive equipment.
    • High sensitivity with a detection threshold as low as 0.5 ng/well for each modification pattern and a detection range from 20 ng to 500 ng/well of histone extracts.
    • An assay control is conveniently included for quantification of each histone H3 modification.
    • Total histone H3 sets are included, which can be used for normalizing total histone H3 levels for relative comparison of histone H3 content between different samples or different treatment conditions.
    • Strip microplate format makes the assay flexible: manual or high throughput, which enables analysis of a single modification or total 21 modification patterns within the same samples.
    • Two extra 8-well strips are included in the kit which can be used, if necessary, for sample amount pre-optimization to determine the input amount (ex: 50, 100, 200 ng/well) needed to fall within the detection limits of the assay. Extra strips may also be used as assay controls and total histone level controls if selective detection of some histone H3 modifications from the total 21 modification pattern is desired.
    • Simple, reliable, and consistent assay conditions.
  • EpiQuik™ CRISPR/Cas9 Assay ELISA Kit
    High throughput measurement of Cas9 levels in purified Cas9 nuclease or whole cell extracts in a microplate-based format. Fast 3-hour procedure time, detection limit as low as 0.1 ng/well, flexible 96 strip-well microplate format with colorimetric assay....

  • The EpiQuik™ CRISPR/Cas9 Assay ELISA Kit (Colorimetric) is suitable for specifically measuring CRISPR-associated protein 9 (Cas9 and dCas9: S. pyogenes) amounts quantitatively with use of purified Cas9 nuclease and whole cell extracts isolated from tissues and cultured cells of various species. The kit has the following advantages:

    • Quick and efficient procedure, which can be finished within 3 hours.
    • Innovative colorimetric assay in high throughput format to quantitatively measure Cas9 protein amount without the need for electrophoresis, especially suitable for screening Cas9-transfected clones. 
    • High sensitivity and specificity. The detection limit is as low as 0.1 ng/well with dynamic range of 0.5-20 ng/well within the indicated amount range of the whole cell extracts. Only recognizes Cas9 (S. pyogenes). 
    • The control is conveniently included for the quantification of Cas9 amount.
    • Strip microplate format makes the assay flexible: manual or high throughput.
    • Simple, reliable, and consistent assay conditions.

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