Biological samples are complex, often containing contaminating nucleic acids from other species. Ribosomal RNA (rRNA) or even common bacterial transcripts are significantly over expressed relative to transcripts of interest. Removal of contaminating or high expressing transcripts will enable better detection of biologically relevant signals. Currently Jumpcode Genomics offers products to enable the following applications:
Whole transcriptome profiling: Remove mammalian rRNA to shift sequencing depth to detection of low abundance RNA’s.
Microbiome: Removal of bacterial rRNA form over 212 bacterial species, covering all phyla in a single depletion reaction. Increase the number of bacterial species detectable by 3-4X.
Infectious Disease Surveillance: Deplete both human and bacterial rRNA sequences from complex biological samples for greater sequencing coverage of viral and bacterial pathogens, and identify low expressing human transcripts related to immune response.