With our scATAC Pre-Indexing Kit, up to 300,000 nuclei can be run in a single experiment. The streamlined workflow integrates tagmentation and sample indexing, enabling users to profile up to 24 samples simultaneously.
Split-pool barcoding and combinatorial indexing methods leverage the cell as a compartment for a simple, instrument-free single-cell workflow as follows:
1. Permeabilized cells are distributed in a multi-well plate where barcoded probes tag cellular analytes with a barcode that is uniform for all cells within a single well.
2. These barcoded cells are pooled, then randomly re-distributed for another round of barcoding.
3. This process is completed when the desired barcode space has been achieved. The number of cells profiled can be scaled up exponentially with repeated rounds of pooling and splitting to enable single-cell experiments with hundreds of thousands to millions of cells.