Fast Core-shell Separation with Nanomolar Detection at a Boron-doped Diamond Electrode

Tuesday, March 09, 2021: 9:50 AM - 10:25 AM

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Description

Combining the nanomolar detectability of solid electrodes with sub-minute high efficiency chromatographic media will present new opportunities in many key applications. In this work, the coupling of electrochemical detection at a boron-doped diamond electrode (BDD) with core-shell chromatography is examined for the rapid nano-molar detection of targeted solutes such as phenolics, neurotransmitters and microbial signalling molecules. The BDD electrode exhibits many advantages, including wide potential range, high current density, excellent electrochemical stability, low background current and high resistance to fouling. Among the key targets are microbial signalling molecules such as those produced by Pseudomonas aeruginosa. This Gram-negative opportunistic pathogen, utilises diffusible signalling molecules, known as ‘autoinducers’ or bacterial pheromones for cell-to-cell communication (quorum sensing (QS)). Critically, it is an antibiotic-resistant human pathogen associated with hospital acquired infections and causes acute pneumonia and chronic lung infections in cystic fibrosis (CF) patients. Early detection is possible through its molecular signature analysis1,2 and is crucial in the clinical management of this pathogen. Rapid LC-BDD analysis of the primary P. aeruginosa AHQ signalling molecules 2-heptyl-3-hydroxy-4-quinolone (“Pseudomonas Quinolone Signal”, PQS) and its immediate precursor, 2-heptyl-4-hydroxyquinoline (HHQ) is reported here. Direct electroanalysis at the BDD electrode is also studied for method optimisation. Application is extended to the detection of guaiacol (2-methoxyphenol), a food and beverage spoilage metabolite indicative of spoilage by Alicyclobacillus spp.

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Additional Info

Keywords: Please select up to 4 keywords ONLY:
Biomedical,Chromatography - Other,Trace Analysis,Voltammetry