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Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: What's Ahead?
Thursday, March 11, 2021: 9:15 AM - 9:50 AM
Speaker(s)
Description
FT-ICR MS inherently offers the highest broadband mass resolution and mass accuracy. FT-ICR mass resolving power varies directly with applied magnetic field strength (NHMFL’s 21 tesla offers the world’s highest field for ICR, and may be tripled by segmented electrode detection). FT-ICR MS is uniquely advantageous for resolution and assignment of elemental compositions for complex mixtures, ranging from petroleum crude oil to top-down proteomics. MS/MS techniques include UV, multiphoton IR, collision-induced, and electron transfer dissociation, with additional enhancements by proton transfer, product ion “parking”, and ultrahigh-resolution precursor isolation by stored waveform inverse FT excitation. Coupling with on-line HPLC increases dynamic range by an order of magnitude. Benchmark performance will be illustrated for applications ranging from environmental oil spills to diagnostic identification of proteoforms in multiple myeloma and hemoglobinopathies. This work was performed at the National High Magnetic Field Laboratory, which is supported by NSF Division of Chemistry through DMR-1644779 and the State of Florida. This work was performed at the National High Magnetic Field Laboratory, which is supported by NSF Division of Chemistry through DMR-1157490 and DMR-1644779 and the State of Florida.
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