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March 8 - 12, 2021

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Virtual Pittcon 2021

Quantification of Peptides with HPLC-FID via Solvent Removal and Catalytic Combustion-Reduction Interface

  • Session Number: L32-01
Wednesday, March 10, 2021: 1:30 PM - 1:50 PM

Speaker(s)

Author
Andrew Jones
Activated Research Company
Co-Author
Dwight Stoll
Gustavus Adolphus College
Co-Author
Tommy Saunders
Activated Research Company

Description

The quantification of peptides and their impurities is often done by HPLC with mass spectrometry or UV detection. These methods, however, are hindered by their varied responses to analytes and impurities, and the difficulty of obtaining standards. Ideally, peptide molecules could be quantified directly and calibrated against a non-peptidic standard. Here, we discuss the quantification of peptides using HPLC coupled with a flame ionization detector (FID) utilizing a new solvent removal interface and catalytic combustion-reduction reactor. We show that the FID provides an equimolar carbon response to several peptides and other analytes, allowing for accurate quantification of peptides on a carbon basis without isotopic standards. The linearity, limit of detection and uniformity of response is compared with several other commercially available detectors. References: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4799795/ https://www.ncbi.nlm.nih.gov/pubmed/25172815/

Additional Info

Keywords: Please select up to 4 keywords ONLY:
Biopharmaceutical,Instrumentation,Proteins and Peptides,Proteomics



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