Biological tissues contain a wide variety of cell types with distinct functions and understanding this heterogeneity at high spatial resolution is of great interest in biomedical research. Immunohistochemistry staining methods are limited to visualizing fewer than 10 protein targets, and the image quality is limited by the specificity of antibodies.... Read More

• Invited Session

New technologies that enable intraoperative tissue analysis are critically needed to guide surgical procedures and improve patient outcomes. In my presentation, I will describe the clinical translation and intraoperative use of the MasSpec Pen technology for direct molecular analysis of in vivo and freshly excised tissues in the operating room.... Read More

• Invited Session

Imaging mass spectrometry (IMS) is a powerful technology that enables the visualization of biochemical processes directly in tissues by combining the molecular specificity of mass spectrometry with the spatial fidelity of microscopic imaging. Especially when studying lipids, there are many isobaric and isomeric molecules that complicate spectral analysis, with each... Read More

• Invited Session

Matrix-assisted laser desorption ionization (MALDI) has matured into a powerful tool for mass spectrometric imaging (MSI) of numerous classes of biomolecules and pharmaceuticals. With this label-free technique, full mass spectra are generated pixel-by-pixel from a matrix-coated surface (e.g., a tissue section), with the help of a finely focused laser allowing... Read More

• Invited Session

Cellular interactions within the tissue micro-environment form the basis of health and disease for all organisms. Exposure to nutrients, toxins, and neighboring cells trigger coordinated molecular responses that impact cellular function and metabolism in a beneficial, adaptive, or detrimental manner. Acquiring molecular information at cellular resolution is thus crucial for... Read More

• Invited Session