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Cost-effective, Continuous Monitoring of Food Safety and Agriculture with Graphene-based Probes
Wednesday, March 10, 2021: 2:05 PM - 2:40 PM
Speaker(s)
Description
Irrigation water is a primary source of fresh produce contamination by bacteria during the preharvest, particularly in hydroponic systems where control of pests and pathogens is a major challenge. In this work, we demonstrate the development of bacteria pathogen biosensor using graphene-based interdigitated electrodes (IDEs). The sensor is incorporated into a particle/sediment trap for real-time analysis of irrigation water in a hydroponic lettuce system. We demonstrate the application of this system using a smartphone-based potentiostat for rapid on-site analysis of water quality. A detailed characterization of the electrochemical behavior was conducted in the presence/absence of DNA and Listeria spp., followed by calibration in various solutions with and without flow. In flow conditions (100 mL samples), the aptasensor had LOD was 48 ± 12 CFU/ mL with a linear range of 10^2 to 10^4 CFU/mL. In stagnant solution with no flow, the aptasensor performance was significantly improved in buffer and hydroponic media. Sensor hysteresis ranged from 2 to 16% after rinsing in a strong basic solution (direct reuse), and was insignificant after removing the aptamer via washing in Piranha solution (reuse after adsorption with fresh aptamer). The aptasensor demonstrated a recovery of 90% and may be reused a limited number of times with minor washing steps. This is the first demonstration of an aptasensor used to monitor microbial water quality for hydroponic lettuce in real time using a smartphone based acquisition system for volumes that conform with the regulatory standards.
Track(s)
Additional Info
Keywords: Please select up to 4 keywords ONLY:
Environmental - Water,Food Safety,Food Science,Agriculture
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