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Conductance Imaging of Epithelial Cell Tight Junctions Effected by Chelating Agents on a Nano-porous Membrane
Monday, March 08, 2021: 8:30 AM - 6:00 PM
Speaker(s)
Description
The recently developed Potentiometric-Scanning Ion Conductance Microscopy (P-SICM), is a rising sub-class of SICM that utilizes a theta pipette that holds micro-electrodes to perform current and potentiometric measurements to produce simultaneous topographical and conductance mapping of biological and non-biological surfaces, which aid in the study of cell monolayer’s permeability. Here, out group presents the quantitative acute permeability effects of the basal side of a Madin-Darby Canine Kidney strain II-Wild Type (MDCKII-WT) cell monolayer on a nano-porous Polyethylene Terephthalate (PET) membrane, in the presence of various concentrations of ethylene glycol-bis(𝛃-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA). Where EGTA has been a well-studied chelating agent for Ca2+ ions that disrupt cell-cell tight junctions (TJs) due to its ability to remove the stability found in these TJs’ cadherins, which are also found within claudin proteins and the E-cadherin/catenin complex. The MDCKII-WT cells serve as a model epithelial cell line, where the study of its permeability will aid in understanding the permeability of epithelial cells found as small molecule and peptide regulating cell barriers found within the epithelia, such as, the blood-brain barrier and its ability to protect the central nervous system from harmful compounds.
Additional Info
Keywords: Please select up to 4 keywords ONLY:
Biotechnology,Membrane,Microscopy,Potentiometry
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