Welcome to your Pittcon 2021 Exhibitor Console!
The Exhibitor Console is your hub for all the information you need to know about exhibiting at Pittcon 2021!
Luminescence-based Bionanosensors for Rapid and Ultrasensitive Detection of Botulinum Neurotoxins Implicated in Foodborne Disease
Wednesday, March 10, 2021: 1:30 PM - 2:05 PM
Speaker(s)
Description
Botulinum neurotoxins (BoNTs) are potent toxins produced by Clostridium bacteria that are responsible for the illness botulism and are listed as bioterrorism agents. Rapid detection and discrimination of BoNT serotypes implicated in foodborne human disease are critical for ensuring timely treatment of patients and identifying sources of toxins. The “gold standard” BoNT detection method, the mouse bioassay, is sensitive and reliable but it is also expensive, slow, and requires the use of live animals. We have developed a nanobiosensor based on Förster resonance energy transfer (FRET) between semiconductor nanocrystals (quantum dots, QDs) and dark quencher-labeled peptide probes that can detect biologically active BoNTs in aqueous media and discriminate the serotypes responsible for foodborne disease. The high sensitivity, simple operation, short detection time, and serotyping capability indicate that the nanobiosensor will be useful for rapid BoNT detection and serotype discrimination in food analysis. Here we present the nanosensor operation and performance benchmarks and report on our ongoing effort to translate this technology to a microfluidic chip-based platform capable of rapidly analyzing food samples in non-laboratory settings.
Additional Info
Keywords: Please select up to 4 keywords ONLY:
Biotechnology,Fluorescence and Luminescence,Food Safety,Nanoscience
For Technical Support with this webpage, please contact support.