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Enrichment and Separation of BRAF Mutant by Out-of plane fICP in a 3D Printed Millifluidic Device for Point-of-care Testing
Monday, March 08, 2021: 8:30 AM - 6:00 PM
Speaker(s)
Description
Point-of-care testing plays an important role in disease diagnosis, especially in areas with limited access to traditional clinical resources. However, the relatively low sensitivity of POC methods, such as lateral flow assays, in combination with low concentration of biomarkers limits their utility. Electrokinetic techniques, such as ion concentration polarization (ICP) focusing can be employed to preconcentrate biomarkers prior to detection. ICP is the simultaneous enrichment and depletion of ions at opposing ends of an ion permselective membrane or bipolar electrode (BPE) when an electric field is applied across it. ICP has proven to be a viable route for separation and enrichment of charged species due to the steep electric field gradient at the boundary of the ion depleted zone. However, fluidic instability along this electric field gradient limits the efficiency of a microfluidic preconcentrator when scaling critical dimensions beyond the microscale.
We address these challenges by employing out-of-plane faradaic ICP (fICP); an approach that uses electrochemical reactions on a 3D electrode to facilitate the ion depletion required for analyte focusing and to improve fluidic stability. Here, we demonstrate that the increased stability afforded by out-of-place fICP facilitates scaling from a microfluidic device to a 3D-printed millifluidic device. Then, devices at both scales are employed for an ICP-enhanced lateral flow assay, in which tumor DNA is enriched within a bioconjugated bead bed, where it hybridizes to a capture probe. The present fICP 3D printed millifluidic device allows for facile fabrication and highly sensitive detection of tumor DNA. Importantly, this device and method can be adapted for detection of other classes of biological analytes such as RNA, antigens, virus particles, and whole bacterial cells, and therefore, has the potential to address many POC diagnostic challenges.
This work is supported by NSF CAREER grant #1849109.
Additional Info
Keywords: Please select up to 4 keywords ONLY:
Fluorescence and Luminescence,Microscopy,Process Analytical Chemistry
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