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March 8 - 12, 2021

ALL TIMES SCHEDULED ARE EASTERN STANDARD TIME (EST)


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Virtual Pittcon 2021

Optimization and Evaluation of Traditional SPME and SPME Arrow for Qualitative Analysis of Meat Aroma

  • Session Number: P054
Monday, March 08, 2021: 8:30 AM - 6:00 PM

Speaker(s)

Co-Author
Alan Owens
GCMS Product Manager
Shimadzu Scientific Instruments, Inc.
Co-Author
Andy Sandy
Applications Scientist
Shimadzu Scientific Instruments, Inc.
Co-Author
Eberhardt Kuhn
Shimadzu Scientific Instruments
Author
Madeleine DiGregorio
Shimadzu Scientific Instruments, Inc.
Co-Author
Nicole Lock
GC Product Manager
Shimadzu Scientific Instruments
Co-Author
Richard Karbowski
Shimadzu Scientific Instruments Inc.

Description

Raw meat on its own has very little aroma, therefore almost all aromas associated with “meatiness” come from the cooking process, which can drastically affect which non-volatile precursors release volatile aroma compounds. Meat substitutes, sometimes called plant-based meat, are products created to resemble meat in both look and taste. A plant protein such as soy protein concentrate, along with colors, stabilizers, and oils, is used to successfully mimic meat flavor and texture. As a result, both meat and meat substitutes are complex matrices to analyze by GCMS without additional sample preparation. Solid phase microextraction (SPME) is a solvent-less extraction technique which makes use of a sorbent fiber to ad/absorb compounds from a headspace or liquid sample. Headspace SPME improves selectivity and sensitivity for volatile compounds and reduces matrix effects. The new SPME Arrow contains a greater quantity of sorbent phase and larger surface area than a traditional fiber, allowing for greater analyte extraction in less time. Combined with a redesigned tip and outer sheath, both throughput and robustness are increased over conventional SPME analyses. This work describes the development and optimization of a SPME-GCMS method suitable for qualitative analysis of cooked-meat aroma, and a comparison of several SPME fibers and the new SPME Arrow. The optimized method is used to profile the aroma of several kinds of cooked-meat substitutes and is then compared against the regular cooked-meat aroma.

Track(s)


Additional Info

Keywords: Please select up to 4 keywords ONLY:
Flavors/Fragrance/Essential Oils,Food Science



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