Rapid Analytical Methods for Quantitative Analysis and Screening for 66 and 225 Residual Pesticide Compounds, Respectively, in Pine Needles and Spices using GCMS Triple Quadrupole

Monday, March 08, 2021: 8:30 AM - 6:00 PM

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Description

Pesticides are indispensable in the protection of agricultural crops and products from pest infestation However, over recent years there has been an increase concern on residual pesticides found in food. Pesticides are ubiquitous through the environment and even low-level concentration can cause alarm due to these compounds acute and chronic toxicity. To that end, it is pivotal that novel instrumentation be able to measure these compounds down to trace concentration, even at the low fg/ul range. The objective of this study is to use GCMS triple quadrupole to conduct quantitative and qualitative analysis of a cocktail of pesticides in sample matrices consisting of a mixture of pine needles and various spices. The study consisted of two sample analyses: 1) a scan analysis to determine over 200 pesticides, and 2) positive EI quantitation of targeted pesticides. Spike samples and standards used were made in 80:20 hexane:toluene. The sample set consisted of 16 vials of extract. The extracts were spiked with 0,10,20 and 50ppb. In this experiment a Shimadzu GCMS-TQ8050NX in conjunction with a ‘Smart Pesticide Database Version 2’ were used for setting up MRM method for positive EI quantification. The pesticide database was also used for development of the scan methods for screening of pesticides. Two hundred and twenty-five pesticides, including isomers, were identified from the screening results. Calibration curves were made in the range of 2.5 to 80ppb for quantitation analysis. Linear responses were obtained from 0.9859 to 0.9999. RF %RSD ranged from 2.892 to 57.495 Most compounds had RF %RSD values less than 30% Results for the EI quantitative analysis are presented in this poster.

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Additional Info

Keywords: Please select up to 4 keywords ONLY:
Method Development,Agriculture