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Ag Nanoparticles Provide New Options for Sensitive Point-of-Care Sensors
Monday, March 08, 2021: 9:10 AM - 9:30 AM
Speaker(s)
Description
Electrochemical immunosensors exhibit many advantages over competing methods. Apart from rapid quantification of the analyte using small sample volumes, high sensitivity and selectivity are ensured; user-friendly handling, simple miniaturization and easy sensor production are easily accomplished. These factors make electrochemical immunosensors highly interesting for clinical applications. Our work focuses on the development of high performance electrochemical biosensors through nanoparticle-mediated signal amplification on carbon-based electrodes. We studied gold (AuNP) and silver (AgNP) nanoparticles as reporter labels detected via differential pulse voltammetry (DPV) in a sandwich immunoassay with the blood protein biomarker NT-proBNP as model analyte using an EmStat3 Blue potentiostat (PalmSens). While AuNPs lend themselves very well for antibody immobilization, DPV detection requires addition of HCl, which is impossible considering true point-of-care applications. In contrast, AgNPs are electrochemically less stable and therefore enable a simplified assay protocol. Interestingly, they also provide a six-times lower LODs of 4.0 ng/mL in buffer and 4.7 ng/mL in human serum, while maintaining the same or even better assay reliability of 17% (n ≥ 4). Thus, in direct comparison, AgNPs clearly outperform AuNPs in desirable point-of-care electrochemical assays and will lead future development of such biosensors. We demonstrated this further through a miniaturized biosensor with a microfluidic setup in which all necessary reagents were dried directly on chip. An excellent LOD of 0.24 ng/mL with a mean error of 9% (n ≥ 3) was reached in buffer, with similar results obtained even in undiluted human serum. This miniaturized biosensor avoids any pipetting step, increases hence reproducibility and assay sensitivity and enables detection below the clinical threshold of 1 ng/mL. Finally, dry biosensor chips can be stored at minimum three months with a signal stability > 85%.
Additional Info
Keywords: Please select up to 4 keywords ONLY:
Flow Injection Analysis,Immunoassay,Voltammetry,Clinical Analysis
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