A Standardized 2DLC Screening Platform for Peak Purity Determination

Monday, March 08, 2021: 8:30 AM - 6:00 PM

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Description

Peak purity analysis of a HPLC chromatogram is a key component in evaluating specificity of a method. The standard method for peak purity determination is comparison of UV spectra obtained via a diode array detector (DAD). Differentiation between purity and impurity is made on the basis of the differences in DAD-acquired spectra throughout the width of a peak in question. For DAD purity to be effective, three conditions must be met: the impurity must be spectrally different from the analyte; there must be some chromatographic resolution between the analyte and the impurity; and the impurity must be present above the limit of detection. Manufacturing impurities and degradants can easily fail the first condition as they are often small changes to a molecule that are distant from the chromophore and therefore result in a similar UV spectrum to the analyte. More often the case is a small impurity with limited resolution beneath an analyte peak in the chromatogram. In both examples, determination of peak purity via DAD inevitably fails to find coeluting impurities. Peak purity analysis via 2DLC is a way to overcome the limitations of peak evaluation via DAD approaches. Subsequent analysis of a peak in the first dimension via an orthogonal method in the second dimension has proven a powerful method of resolving impurities quickly and with little downtime or secondary analysis (fraction collecting, MS, etc.). This work details a standardized 2DLC screening method for peak purity determination that has been utilized to resolve impurities that DAD detection fails to capture.

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Additional Info

Keywords: Please select up to 4 keywords ONLY:
Chromatography - Other,Method Development,Process Analytical Chemistry