Virtual Event
March 8 - 12, 2021


HPLC and UHPLC for Practicing Scientists 1: Fundamentals [FREE TEXTBOOK Included] (PART 1 of 2)

  • (Full Day Course - Part 1 & 2 are required together)
  • Course Number:SC6717A
Thursday,March 04, 2021:8:30 AM -12:30 PM


Michael Dong
MWD Consulting


"FREE TEXTBOOK Included" This intermediate 1-day workshop will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, and columns of HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on pharmaceutical analysis of small molecule drugs.

Register for this Short Course

Course Outline

Day 1: HPLC 1: Fundamentals

1. Concepts

A. Introduction: The chromatographic process, advantages and limitations, and chromatographic modes

B. Concepts: Retention factor (k), separation factor, column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM)

C. Mobile phase factors, operating parameters, column temperature (T)), peak capacity (Pc)

2. HPLC Columns, Trends and Selection Guides

A. Column fundamentals: Glossary, bonded phases, plate height (H), the van Deemter equation

B. Trends: the use of high-purity silica, column selection guide

C. Newer column types: Hybrids, sub-3 and sub-2um, and superficially porous particles (SPP)

3. HPLC instrumentation and Operating Principles

A. The system, Pump, and Autosampler.

B. Detectors: Operating principles of UV/Vis detection and characteristics

C. MS, CDS, and Instrument Bandwidth.

Additional Info

Course Sub-Categories (Up to 5):
Data Analysis,Liquid Chromatography,Liquid Chromatography-Mass Spectrometry,Pharmaceutical Sciences,Quality/Regulatory/Compliance

Proposal Number:

Target Audience:
Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the fundamentals of HPLC and UHPLC in pharmaceutical analysis and other applications. A basic understanding of chemistry and HPLC with some hands-on experience is assumed