Separations: Fundamentals of Gel Permeation & Size Exclusion Chromatography with Advanced Detection
- (Half Day Course)
- Course Number:SC7836
Friday,March 05, 2021:1:00 PM -5:00 PM
This ½ day course covers the theoretical and practical principles of GPC & SEC. We will discuss how light scattering overcomes the limitations of column calibration to provide a direct measurement of ‘absolute’ molecular weight, irrespective of the sample’s chemistry, composition, and structure. This course will discuss the principles behind the different advanced detectors and how sample molecular weight and other parameters can be measured. We will also discuss practical aspects of making high quality GPC/SEC measurements and generating consistent results. Finally, we will look at a series of application examples to see how these measurements can be applied to a wide range of applications and samples types.
Register for this Short Course
(15min) Introduction & Course Objectives: What is GPC/SEC?
(30min) Fundamental and Limitations of the Conventional/single-detector GPC/SEC
(30min) Principles of molecular weight measurements by light scattering
(30min) Introduction to intrinsic viscosity, its measurement and meaning
15min) Coffee/Tea Break
(45min) Practical considerations of multi-detector GPC/SEC measurements
(45min) Case Studies of Applications in Polymers, Biopolymers & Proteins
(30min) Practical Take Home Messages, Question and Answer Session
Course Sub-Categories (Up to 5):
Analytical Metrology,Life Sciences,Liquid Chromatography,Pharmaceutical Sciences,Polymers,Quality/Regulatory/Compliance
Those with some general practical GPC or SEC experience who want to maximize the value obtained from their existing equipment and those who are considering the potential benefit of adding advanced detectors to their chromatography characterization. Scientists, R&D personnel, Laboratory Technicians, QA/QC.