A New Dual Range (C-H/FP) QCL for Optical Photothermal Infrared (O-PTIR) Spectroscopy – A New Paradigm in Vibrational Spectroscopy
Wednesday, March 10, 2021: 3:45 PM - 4:05 PM
The recent advent of Optical Photothermal IR (O-PTIR), has enabled for the first time, truly submicron infrared microscopy in far-field reflection mode providing “FTIR transmission-like” spectral quality, without the typical reflection associated spectral artefacts and distortions such as Mie Scattering often associated with traditional FTIR or other emerging QCL IR microscopy systems. With QCL technology ever improving, we present here the first O-PTIR based application of a new Dual Range (C-H/FP) QCL, covering the important C-H stretching and fingerprint regions (3000-2700, 1800-950cm-1) in a single device. The fundamental basis for the technique, the “IR Photothermal Effect” is not new and has been exploited for decades with techniques such as PhotoAcoustic Spectroscopy (PAS) and more recently with AFM-IR (nano-IR). Where O-PTIR differs to these other Photothermal techniques is that it uses an optical (green laser) probe for detection, being analogous to the microphone in PAS and the AFM tip in AFM-IR. The use of this optical probe is the key enabling breakthrough in O-PTIR allowing for non-contact, far-field measurements, which provides for numerous benefits in instrument capabilities but also in instrument architecture, thus allowing for a combined (correlative) IR and Raman (IR+Raman) platform that provides for simultaneous IR and Raman spectral information at the same time, from the same spot with the same submicron spatial resolution. These unique and exciting synergistic capabilities are now spawning interest in life science application. In this presentation, examples of life science applications, ranging from live cell imaging with submicron resolution of organelles, to intracellular imaging of cells on glass using the new Dual Range (C-H/FP) QCL, to ultra-high resolution images of breast tissue calcifications as well as IR+Raman microscopy of microplastics through to polymeric examples showing phase distribution resolution of up to 250nm will be given.
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